Summary: Interest in molecular beacon probes is rapidly increasing.
It has been known that molecular beacons have started arousing interest due their continuous readout, and outstanding spatial resolution when observed in real time. These kinds of dual-labeled probes have the ability to differentiate between two types of bound and unbound RNA and DNA in living cells. Furthermore, they have been used as research tools to sense both DNA and RNA. Now, there are both advantages and disadvantages when using these probes as a method for detecting nucleic acids.
Nucleic acids play a pivotal role in biological activity. They have also helped researchers study the evolution of biology, the analysis of genes, and disease diagnosis. Furthermore, oligonucleotides that have a unique type of sugar backbone can also be replicated in vitro to achieve excellent sensitivity and selectivity in regards to synthesis technology.
The Role of Molecular Beacon Probes
A large number of molecular beacon probes that have been produced in the laboratory can be used to treat a wide array of analysis with high affinity. This goes on to prove that oligonucleotide probes that have different sugar backbones can be used in the detection of nuclear acid. The two most studied XNAs currently being looked at are peptide nucleic acid and locked nucleic acid. This is not to say that other XNAs do not play an important role in the field of nucleic acids, as they largely impact how nuclear acids function. The use of a molecular beacon probe in conjunction with nucleic acids has helped researchers identify and quantify their roles and relationships with DNA and RNA.